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Template:Team:UC Berkeley/Notebook/CC construction
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Contents |
K112501
Construction of {<HA>} basic part K112501
Wobble PCR of cc001/cc002 ( 60 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112501
--------------------------------------------
cc001 Forward Biobricking of {<HA>}
CgATAgaattcATGagatcttacccatacgacgtcccagac
cc002 Reverse biobricking of {<HA>}
ccagtggatccccagcgtagtctgggacgtcgtatggg
K112502
Construction of {<HA!} basic part K112502
Wobble PCR of cc001/cc003 (___bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112502
--------------------------------------------
cc001 Forward Biobricking of {<HA>}
CgATAgaattcATGagatcttacccatacgacgtcccagac
cc003 Reverse biobricking of {<HA!}
ccagtggatccttaccagcgtagtctgggacgtcgtatgggtaag
K112503
Construction of {<myc>} basic part K112503
Wobble PCR of cc004/cc005 (___bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112503
--------------------------------------------
cc004 Forward Biobricking of {<myc>}
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
cc005 Reverse Biobricking of {<myc>}
ccagtggatccCAGATCCTCTTCTGAGATGAGTTTTTG
K112504
Construction of {<myc!} basic part K112503
Wobble PCR of cc004/cc006 (___bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112504
--------------------------------------------
cc004 Forward Biobricking of {<myc>}
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
cc006 Reverse Biobricking of {<myc!}
ccagtggatccTTACAGATCCTCTTCTGAGATGAGTTTTTGTTC
