EPF-Lausanne/20 October 2008

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Cloning

  • Cloning of BC1 into AB
    • Using the simplified ligation protocol.
  • Cloning of CD into LuxI+ (LuxI with RBS and Term.)
    • Here a modified version of the ligation protocol was used, because the insert is quite big (over 2kb).
    • Reaction mix:
      • 3μl vector
      • 5.5μl insert
      • 1μl Ligase Buffer with ATP
      • 0.5μl T4 Ligase

=> Cloning worked.

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