Team:Chiba/Calendar-Home/27 August 2008

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26 August 2008 <|> 28 August 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

Digestion Test

Sample DNA :[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
Sample No. 123
Sample DNA 111
PstI --0.5
EcoRI -0.50.5
Buffer 3 -11
BSA --1
dH2O -7.52
TOTAL 1μl10μl10μl


--->Gel Check
Chiba-0827.JPG
Sample No. 123
Sample DNA 155
Loading Dye 111
H2O 4--
TOTAL 6μl6μl6μl
From left;
  1. [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
  2. Single Digestion
  3. Double Digestion


(26/8)--->Colony-PCR
Colony PCR of 5 colonies from ligation plates (26/8-(4),(5)) and one from control plate([http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)).
DNA Template 1
dNTP mix 5
Foward Primer 5
Reverse Primer 5
DNA polymerase 0.5
Thermopol Buffer 5
dH2O 28.5
TOTAL 50μL


95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C



--->Gel Check
Chiba-0827-2.JPG
Sample DNA 10
Loading Dye 2
TOTAL 12μl
From left;
1~5 insert:[http://partsregistry.org/Part:BBa_C0170 BBa_C0170] + vector:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500] -> OK
6 [http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)-->Positive Control -> OK
7~11 insert:[http://partsregistry.org/Part:BBa_C0178 BBa_C0178] + vector:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500] -> OK
--->(28/8)Miniprep


Transformation

competent cells : XL10G
  • [http://partsregistry.org/Part:BBa_C0062 BBa_C0062](2007)
  • [http://partsregistry.org/Part:BBa_C0062 BBa_C0062](2006) -> no colony on plates
  • [http://partsregistry.org/Part:BBa_C0179 BBa_C0179](2007)
  • [http://partsregistry.org/Part:BBa_C0179 BBa_C0179](2006)
  • [http://partsregistry.org/Part:BBa_S03156 BBa_S03156](2007)
  • [http://partsregistry.org/Part:BBa_S03156 BBa_S03156](2006)
  • [http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2007)
  • [http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2006)
  • [http://partsregistry.org/Part:BBa_S03160 BBa_S03160](2007)
  • [http://partsregistry.org/Part:BBa_S03160 BBa_S0316](2006)


--->Mini prep


--->(29/8)Gel Check

Team:Output

no work

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