User:University of Washington/25 June 2008

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Yeast Shuttle Plasmid

-Obtained plate of yeast strain BY4741 (MATalpha, ΔBar1::Kan MX, his3Δ1; leu2Δ0; met15Δ0; ura3Δ0)

-Incubated yeast at 30 degrees Celsius overnight


LuxR under AraC and TetR

-Obtained BBa_R0040 (TetR), BBa_R0080 (AraC)

-transformed and plated with Amp

LuxR under AraC and TetR

-Obtained parts R0010, I0462, and J04430. -R0010 and I0462 were transformed into MG strains, and plated on Amp. -J04430 was transformed into DH strain, and plated on Amp.


Team:University_of_Washington/Notebook#Notebook

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