"
Minnesota/23 June 2008
From 2008.igem.org
| Back to Notebook | |
| Go to Previous Day (June 20) | Go to Next Day (June 24) |
| 1. Continued plasmid prep work |
| 2. Look up available Biobrick Plasmids |
| 3. Begin iGEM wiki |
| 4. Perform double digest. Double digest is when the plasmid is cut at two different restriction sites by using restriction enzymes. |
| Double Digest | 4x | 10x | |
|---|---|---|---|
| 1.0 micrograms DNA | 2 | 1 | |
| (10x) Buffer 2 | 20 | 50 | |
| (100x) BSA | 2 | 5 | |
| (20 units/uL) XBA1 | 2 | 5 | |
| (10 units/uL) Spe1 | 4 | 10 | |
| H20 | 164 | 420 | |
| Total sln | 50 uL | 50 uL |
