From 2008.igem.org
Molly's Construction Files
Molly's Notebook
Construction of {xis!} Biobrick Part K112200
PCR mea001/mea002 on pAH57 (230 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112200
----------------------------------------
mea001 Forward Biobricking of {xis!} ccataGAATTCatgAGATCTatgtacttgacacttcaggag
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttccc
Construction of {<xis!} Biobrick Part K112201
PCR mea003/mea002 on pAH57 (227 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112201
----------------------------------------
mea003 Forward Biobricking of {<xis!} ccataGAATTCatgAGATCTtacttgacacttcaggagtg
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttccc
Construction of {xis>} Biobrick Part K112202
PCR mea001/mea004 on pAH57 (227 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112202
----------------------------------------
mea001 Forward Biobricking of {xis!} ccataGAATTCatgAGATCTatgtacttgacacttcaggag
mea004 Reverse Biobricking of {xis>} cgttaGGATCCtgacttcgccttcttcccatttc
Construction of {<xis>} Biobrick Part K112203
PCR mea003/mea004 on pAH57 (224 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112203
----------------------------------------
mea003 Forward Biobricking of {<xis!} ccataGAATTCatgAGATCTtacttgacacttcaggagtg
mea004 Reverse Biobricking of {xis>} cgttaGGATCCtgacttcgccttcttcccatttc
Construction of {a~xis!} Biobrick Part K112204
PCR mea017/mea002 on pAH57 (234 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112204
----------------------------------------
mea017 Forward Biobricking of {a~xis!}ccagtGAATTCatgAGATCTtgcgatgtacttgacacttcagg
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttccc
Construction of {rbs.xis!} Biobrick Part K112205
PCR mea018/mea002 on pAH57 (249 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112205
----------------------------------------
mea018 Forward Biobricking of {rbs.xis!}ccagtGAATTCatgAGATCTtaattgcggagactttgcgatg
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttccc
Construction of {int!} Biobrick Part K112207
PCR mea005/mea006 on pAH57 (1102 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112207
----------------------------------------
mea005 Forward Biobricking of {int!} ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc
Construction of {<int!} Biobrick Part K112208
PCR mea007/mea006 on pAH57 (1099 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112208
----------------------------------------
mea007 Forward Biobricking of {<int!} ccataGAATTCatgAGATCTggaagaaggcgaagtcatgagc
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc
Construction of {int>} Biobrick Part K112209
PCR mea005/mea008 on pAH57 (1099 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112209
----------------------------------------
mea005 Forward Biobricking of {int!} ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg
mea008 Reverse Biobricking of {int>} cgttaGGATCCtttgatttcaattttgtcccactccc
Construction of {<int>} Biobrick Part K112210
PCR mea007/mea008 on pAH57 (1096 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112210
----------------------------------------
mea007 Forward Biobricking of {<int!} ccataGAATTCatgAGATCTggaagaaggcgaagtcatgagc
mea008 Reverse Biobricking of {int>} cgttaGGATCCtttgatttcaattttgtcccactccc
Construction of {a~int!} Biobrick Part K112211
PCR mea019/mea006 on pAH57 (1106 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112211
----------------------------------------
mea019 Forward Biobricking of {a~int!}ccataGAATTCatgAGATCTagaaatgggaagaaggcgaag
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc
Construction of {rbs.int!} Biobrick Part K112212
PCR mea020/mea006 on pAH57 (1120 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112212
----------------------------------------
mea020 Forward Biobricking of {rbs.int!}ccataGAATTCatgAGATCTttttgaagaggatcagaaatggg
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc
Construction of {ihfA!} Biobrick Part K112213
PCR mea009/mea010 on MG1655 (331 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112213
----------------------------------------
mea009 Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagcg
Construction of {<ihfA!} Biobrick Part K112214
PCR mea011/mea010 on MG1655 (328 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112214
----------------------------------------
mea011 Forward Biobricking of {<ihfA!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagcg
Construction of {ihfA>} Biobrick Part K112215
PCR mea009/mea012 on MG1655 (328 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112215
----------------------------------------
mea009 Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg
mea012 Reverse Biobricking of {ihfA>} cgttaGGATCCctcgtctttgggcgaagcg
Construction of {<ihfA>} Biobrick Part K112216
PCR mea011/mea012 on MG1655 (325 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112216
----------------------------------------
mea011 Forward Biobricking of {<ihfA!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc
mea012 Reverse Biobricking of {ihfA>} cgttaGGATCCctcgtctttgggcgaagcg
Construction of {a~ihfA!} Biobrick Part K112217
PCR mea021/mea010 on MG1655 (335 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112217
----------------------------------------
mea021 Forward Biobricking of {a~ihfA!}ccataGAATTCatgAGATCTacctatggcgcttacaaaagc
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagcg
Construction of {rbs.ihfA!} Biobrick Part K112218
PCR mea022/mea010 on MG1655 (350 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112218
----------------------------------------
mea022 Forward Biobricking of {rbs.ihfA!}ccataGAATTCatgAGATCTtcattgagggattgaacctatggcgc
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagcg
Construction of {ihfB!} Biobrick Part K112219
PCR mea013/mea014 on MG1655 (316 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112219
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mea013 Forward Biobricking of {ihfB!} ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagacttgccaccc
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
Construction of {<ihfB!} Biobrick Part K112220
PCR mea015/mea014 on MG1655 (313 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112220
----------------------------------------
mea015 Forward Biobricking of {<ihfB!} ccataGAATTCatgAGATCTaccaagtcagaattgatagaaagacttgccaccc
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
Construction of {ihfB>} Biobrick Part K112221
PCR mea013/mea016 on MG1655 (313 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112221
----------------------------------------
mea013 Forward Biobricking of {ihfB!} ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagacttgccaccc
mea016 Reverse Biobricking of {ihfB>} cgttaGGATCCaccgtaaatattggcgcgatc
Construction of {<ihfB>} Biobrick Part K112222
PCR mea015/mea016 on MG1655 (310 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112222
----------------------------------------
mea015 Forward Biobricking of {<ihfB!} ccataGAATTCatgAGATCTaccaagtcagaattgatagaaagacttgccaccc
mea016 Reverse Biobricking of {ihfB>} cgttaGGATCCaccgtaaatattggcgcgatc
Construction of {a~ihfB!} Biobrick Part K112223
PCR mea023/mea014 on MG1655 (320 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112223
----------------------------------------
mea023 Forward Biobricking of {a~ihfB!}ccataGAATTCatgAGATCTaatcatgaccaagtcagaattgatag
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
Construction of {rbs.ihfB!} Biobrick Part K112224
PCR mea024/mea014 on MG1655 (337 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112224
----------------------------------------
mea024 Forward Biobricking of {rbs.ihfB!}ccataGAATTCatgAGATCTctttaaggaaccggaggaatcatg
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
Construction of {ompT>} Biobrick Part K112225
Wobble PCR mea025/mea026 (97 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112225
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mea025 Forward Biobricking of {ompT>} cgataGAATTCatgAGATCTatgcgggcgaaactcctaggaatagtcctgacaacccctatcg
mea026 Reverse Biobricking of {ompT>} cgttaGGATCCcgtcgacgcaaaagagctgatcgcgataggggttgtcaggactattc
Construction of {a~ompT} Biobrick Part K112226
Wobble PCR mea027/mea026 (101 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112226
----------------------------------------
mea027 Forward Biobricking of {a~ompT} cgataGAATTCatgAGATCTacatatgcgggcgaaactcctaggaatagtcctgacaacccctatc
mea026 Reverse Biobricking of {ompT>} cgttaGGATCCcgtcgacgcaaaagagctgatcgcgataggggttgtcaggactattc
Construction of {rbs.ompT} Biobrick Part K112227
Wobble PCR mea028/mea026 (114 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112227
----------------------------------------
mea028 Forward Biobricking of {rbs.ompT}cgataGAATTCatgAGATCTaagaaggagatatacatatgcgggcgaaactcctaggaatagtcctgacaacccc
mea026 Reverse Biobricking of {ompT>} cgttaGGATCCcgtcgacgcaaaagagctgatcgcgataggggttgtcaggactattc
Construction of {pelB>} Biobrick Part K112228
Wobble PCR mea029/mea030 (97 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112228
----------------------------------------
mea029 Forward Biobricking of {pelB>} cgataGAATTCatgAGATCTatgaaatacctgctgccgaccgctgctgctggtctgctgctcc
mea030 Reverse Biobricking of {pelB>} cgttaGGATCCggccatcgccggctgggcagcgaggagcagcagaccagcagcagcgg
Construction of {a~pelB} Biobrick Part K112229
Wobble PCR mea031/mea030 (101 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112229
----------------------------------------
mea031 Forward Biobricking of {a~pelB} cgataGAATTCatgAGATCTacatatgaaatacctgctgccgaccgctgctgctggtctgctg
mea030 Reverse Biobricking of {pelB>} cgttaGGATCCggccatcgccggctgggcagcgaggagcagcagaccagcagcagcgg
Construction of {rbs.pelB} Biobrick Part K112230
Wobble PCR mea032/mea030 (116 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112230
----------------------------------------
mea032 Forward Biobricking of {rbs.pelB} cgataGAATTCatgAGATCTttaagaaggagatatacatatgaaatacctgctgccgaccgctgctgctggtctgctg
mea030 Reverse Biobricking of {pelB>} cgttaGGATCCggccatcgccggctgggcagcgaggagcagcagaccagcagcagcgg
Construction of {<barstar!} Biobrick Part K112231
PCR mea033/mea034 on Bacillus amyloliquefaciens gen.
(301 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112231
----------------------------------------
mea033 Forward Biobricking of {<barstar!} cgataGAATTCatgAGATCTaaaaaagcagtcattaacgggg
mea034 Reverse Biobricking of {<barstar!} cgttaGGATCCttaagaaagtatgatggtgatgtcgc
Construction of {rbs.barstar!} Biobrick Part K112232
PCR mea035/mea034 on Bacillus amyloliquefaciens gen.
(320 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112232
----------------------------------------
mea035 Forward Biobricking of {rbs.barstar!} cgataGAATTCatgAGATCTcataagaaaggagccgcacatg
mea034 Reverse Biobricking of {rbs.barstar!} cgttaGGATCCttaagaaagtatgatggtgatgtcgc
Construction of {<phoA>} Biobrick Part K112233
PCR mea035/mea034 on ??????? (1219 bp, EcoRI/BamHI)
Sub into pBca9145 (EcoRI/BamHI, 2057+696, L)
Product is pBca9145-K112233
----------------------------------------
mea036 Forward Biobricking of {<phoA>} cgataGAATTCatgAGATCTggagattctgaaataaccgccg
mea037 Reverse Biobricking of {<phoA>} cgttaGGATCCcttcaggcccagcgccgc
Molly's Notebook
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