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Team:University of Lethbridge/Notebook/Project1July
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July 1, 2008
Nathan Puhl
Subcultured RP1616 into liquid LB and pTopp cells into liquid LB + amp
July 2, 2008
Nathan Puhl, Christa, Munima
Plasma mini prepped pTopp cells
Attempted to make RP1616 cells competent using the open wet ware [http://openwetware.org/wiki/Preparing_chemically_competent_cells protocol]. 100 uL aliquots of cells were froxen in liquid nitrogen and stored in the -80 freezer.
Ran CheZ in pUC19 plasmid and pUC19 plasmid from last year with high range ladder. The pUC19 plasmid seems weird, I don't think it should have two bands; I will have to ask someone about that. The CheZ plasmid appears to be the right size (2600 + 620 = 3220 bp)
