Rensselaer/7 July 2008

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Overviewed procedure for introducing gene of interest:

pick plasmid filter paper - into buffer primer design - insert restriction site (comparable with plasmid) PCR (primers, DNA polymerase, dNTP's,buffer) Digestion w/ restriction enzymes Ligation (plasmid and insert) introduce to bacteria (transformation)

potential problem - psuedomonas - how to get plasmid DNA?

perform PCR on solution