Team:NTU-Singapore/Notebook/9 July 2008

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E7 Story continued...

  • Ethidium Bromide Gel showed clean bands at 2k mark, took PCR direct to PCR purified to 30ul.
    • Gel run-smeary band..S*** What went wrong?
  • Trouble SHOOT SHOOT
    • Protocol different from 8 July
      • Optimize Protocol

HOW? Take Gel extracted E7(50ul) direct, Gel extract(50ul)-PCR Purified to run gel to see the difference..To see the effectiveness of PCR purification and Gel Extract..Compare!

T7ptag Story 2

PCR product obtained..Ran Gel with 4ul..Good clear band Ran gel to purify the rest..loaded with 10ul

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