Team:ESBS-Strasbourg/Protocols

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E.Coli

Preparation of competent cells (Mariels/Barbaras Protocol) Competent Bacteria

Preparation of TOP10 chemically competent cells [http://openwetware.org/wiki/TOP10_chemically_competent_cells igem standard protocol]

Transformation of competent cells (Mariels/Barbaras Protocol) Transformation

Cloning

PCR
Purification of PCR product
Miniprep: plasmid amplification
Plasmid Purification
Restriction digestion
Agarose gel preparation
Dephosphorylation
Ligation

Solutions and buffers

SOB for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB]

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