Paris/July 11

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yesterday -- tomorrow



Cultures

The strains stocked at -80°C were taken out and spread (streaks) on LB boxes (without antibiotic). Boxes have been put in the steam room at 37°C at 15h45


Code Strain
DW 27 MG1655 ΔfliA::Cm
NEC 283 MG 1655 ΔfliA::FRT
NEC 284 MG 1655 ΔflhD::FRT
DW 26 MG 1655 ΔflgM::Cm
NEC 285 MG 1655 ΔflgM::FRT
DW 35 JW K1879 ΔmotA::Kan
NEC 288 MG 1655 ΔmotA::FRT
DW 37 JW K1068 ΔflgJ::Kan
NEC 287 MG 1655 ΔflgJ::FRT
DW 24 MG 1655 ΔfliC::Cm
NEC 286 MG 1655 ΔfliC::FRT

Manipulations

  • Culture of DH5ɑ in 5mL of LB
  • Preparation of 250mL of CaCl2 0,1M
  • Transfer of the equipment iGEM 07 on the sixth floor
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