Team:Paris/July 14

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Results of the transformation

Positive control : puc19 (1µl; 10pg/ml) --> No transformation
Biobricks 2007 : --> succeed of the transformation unlike the delay added in the ice
Biobricks 2008 : --> No transformation

          Conclusion : Cells are competents, because there is positive transformation
          Hypothesis : problem with the protocol of DNA extraction from the registery 2008

Culture of Cells

Culture of DH5ɑ in 5ml of LB without Antibiotics

Preparation of medium and solutions

1L of CCMB80
500ml of SOB ( 2 x 250ml)
500ml of SOC (2 x 250ml)
100ml of MgCl₂ (1M)
150ml of CaCl₂ (1M)
200ml of KOAC (1M)