Team:University of Ottawa/15 July 2008

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Today on the Lab

Tammy Transformation of XL10 Competent E. coli cells with pDR197:AtCKX2

  • 1 uL Pure pDR197:AtCKX2
  • 1 ul 1:10 dilution pDR197:AtCKX2
  • 1 ul 1:100 dilution pDR197:AtCKX2
  • 100 ul XL10 E.Coli per reaction
  • 900 ul LB Broth per reaction
  • 3 LB Ampicillin (50 ug/mL) Plates
  • Began Incubation of transformed cells at 1:50 pm.

    • Matt Inoculation of 97 - BY4742 cells

  • I inoculated the 97 BY4742 cells once again for glycerol stock tomorrow

    • PCR confirmation

  • Second PCR confirmation with F58, F59 was performed and gave the correct band but also gave another weird band that I don't know what it is.

    • Digestion of PTP2

  • A digestion was performed on the PTP2 amplification product to create sticky ends using BamHI and xhoI and I left it in the PCR machine overnight.
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