Minnesota/17 July 2008

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1. Plasmid prep dual promoters: (1) Tet & P22mnt, (2) LacI & LAMBDAcI.
2. Double digest of all ligations: from previous days.
3. Sequence L5, L6-L9
4. Re-transform RFP, TetR promoter, terminator because no cell growth on plates.
5. Discuss problems with sequences
6. Model: ?? Why GFP output is low???