From 2008.igem.org
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DryLab
Modeling
WetLab
- Results Transformation
- no clones for any of the three BioBricks
- clones for the LexA-BD plasmid --> useable for Miniprep
- counting:
- dh5a (Mariel) + pxj - KCM : 10^5 cfu/µg
- dh5a (Mariel) + pxj - HS : 4*10^4 cfu/µg
- dh5a + pxj - HS : 1.5*10^5 cfu/µg
- Top10 + pxj - HS : 3.2*10^5 cfu/µg
- db3.1 + pxj - HS : 3*10^4 cfu/µg
- Digestion of the plasmid of the BBa_J63002(?) clone with XbaI and SpeI
- 1x double digestion
- 1x step by step digestion
General
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