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21 May 2008
From 2008.igem.org
Volunteers Scheduled for Today
5-8 AS
6-8 DL
Things to do:
Mini-preps of O/N's
Sequence gel purified fragments from yesterday
Transform I0500
Digest J61003 with Xba/Pst to insert ORF's
Digest minipreps with Xba/Pst to insert into J61003
Run gel of digests and excise correct fragments
Ligate vector/inserts
Transform ligation reactions
Lab clean up
The Good and Bad news
The Good News
Our system will work in E.coli
The Bad News
In 2004 Texas submitted the cyanbacterial equivalent of our system, so... Our biobricks would be unique but they would do the exact same thing.
The Good News
I looked up a very promising pathway that detects blue light instead
The Bad News
I can only find a summary of the paper... Here is the link to it
http://www.arabidopsis.org/servlets/TairObject?type=publication&id=501724582
