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Team:University of Ottawa/21 July 2008
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Today in the lab
Dan
- Gel of sample 2T
- For some odd reason all of the DNA stayed in the well... Could be some sort of salt contamination from NEB buffer 1, or a very large autoligation product. I will test the former by doing PCR cleanup and switching to ligation buffer and then run the gel again.
- Digestion of 1A 0B and both S and D
- The above DNA fragments were digested with PstI and SphI in Neb buffer 1, enzymes were denatured after an hour
- PCR cleanup was performed on the digested products, eluted with 50 uL water.
- Ligation
- Ligation of the DNA obtained from PCR cleanup was run overnight at 16 C
