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Team:KULeuven/23 July 2008
From 2008.igem.org
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Lab Work
Wet Lab
Stefanie and I prepared wet cultures from the plates with the cells we received from iGEM. We also started on the glycerol stock. Tomorrow we'll continue and make a glycerol stock of all the parts. The other team started with restriction and ligation of the output.
Dry Lab
One big question: is the stop codon in the scar a problem in linking a tag and a protein coding region? The BioBricks of [http://partsregistry.org/wiki/index.php?title=Part:BBa_I712043 Ljubljana] prove not, but is this correct?
Modeling
Modeling wiki has been updated, Output, Cell Death, Inverter, Memory, Filter, Pulse Generator(still need for a parameter check) have been revisited, added graphs of both CellDesigner and Matlab. A pdf file containing the ODE equations and more was made.
I'm affraid we will have to come back on our decision to simply remove the pulsgenerator and put the lactonase production after the filter. (todo: completion)
