Team:The University of Alberta/24 July 2008

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Revision as of 20:14, 24 July 2008 by Cwk (Talk | contribs)

General

  • Did mini-preps of J61003 because we were running out
  • Digested BisDA/B with single digests using AgeI and NgoMIV to test the enzymes because weve been getting cruddy digests when using both of them

System 2

Today we have taken the ligation from yesterday and transformed it as well as digested it.

  • if all goes well the digested product should be B0034+TETR
    • Column Purified the digested product.
  • we will then ligate the B0034+TETR with LacIQERE giving us a complete system 2.
  • Digested pTet with Spe/Pst.
  • Digested RFP with Xba/Pst.

System 3

  • Digested 5ul of J61003 with Xba/Pst; this will let us insert the Tryp biobrick digested and ligated yesterday
    • Ran the digested J6 on a gel; gel purified
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