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Team:KULeuven/29 July 2008
From 2008.igem.org
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Lab Work
Wet Lab
Only one ligation succeeded (R0084+B0032). We think that the others failed because the vectors weren't properly cut. Therefore, we will try another digest. This time we will cut the vector with Xba I, and only when this works, we will cut it with EcoR I. We do this because Xba I has problems with cleaving close to the end of DNA fragments.
