Team:EPF-Lausanne/Notebook

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Notebook

You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.

Find more information on how to use the calendar feature by going to the general calendar page.

April
M	T	W	T	F	S	S
	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/1_April_2008&action=edit 1]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/2_April_2008&action=edit 2]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/3_April_2008&action=edit 3]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/4_April_2008&action=edit 4]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/5_April_2008&action=edit 5]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/6_April_2008&action=edit 6]
[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/7_April_2008&action=edit 7]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/8_April_2008&action=edit 8]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/9_April_2008&action=edit 9]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/10_April_2008&action=edit 10]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/11_April_2008&action=edit 11]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/12_April_2008&action=edit 12]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/13_April_2008&action=edit 13]
[http://2008.igem.org/EPF-Lausanne/14_April_2008 14]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/15_April_2008&action=edit 15]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/16_April_2008&action=edit 16]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/17_April_2008&action=edit 17]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/18_April_2008&action=edit 18]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/19_April_2008&action=edit 19]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/20_April_2008&action=edit 20]
[http://2008.igem.org/EPF-Lausanne/21_April_2008 21]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/22_April_2008&action=edit 22]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/23_April_2008&action=edit 23]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/24_April_2008&action=edit 24]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/25_April_2008&action=edit 25]	[http://2008.igem.org/EPF-Lausanne/26_April_2008 26]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/27_April_2008&action=edit 27]
[http://2008.igem.org/EPF-Lausanne/28_April_2008 28]	[http://2008.igem.org/EPF-Lausanne/29_April_2008 29]	[http://2008.igem.org/EPF-Lausanne/30_April_2008 30]

May
M	T	W	T	F	S	S
			[http://2008.igem.org/EPF-Lausanne/1_May_2008 1]	[http://2008.igem.org/EPF-Lausanne/2_May_2008 2]	[http://2008.igem.org/EPF-Lausanne/3_May_2008 3]	[http://2008.igem.org/EPF-Lausanne/4_May_2008 4]
[http://2008.igem.org/EPF-Lausanne/5_May_2008 5]	[http://2008.igem.org/EPF-Lausanne/6_May_2008 6]	[http://2008.igem.org/EPF-Lausanne/7_May_2008 7]	[http://2008.igem.org/EPF-Lausanne/8_May_2008 8]	[http://2008.igem.org/EPF-Lausanne/9_May_2008 9]	[http://2008.igem.org/EPF-Lausanne/10_May_2008 10]	[http://2008.igem.org/EPF-Lausanne/11_May_2008 11]
[http://2008.igem.org/EPF-Lausanne/12_May_2008 12]	[http://2008.igem.org/EPF-Lausanne/13_May_2008 13]	[http://2008.igem.org/EPF-Lausanne/14_May_2008 14]	[http://2008.igem.org/EPF-Lausanne/15_May_2008 15]	[http://2008.igem.org/EPF-Lausanne/16_May_2008 16]	[http://2008.igem.org/EPF-Lausanne/17_May_2008 17]	[http://2008.igem.org/EPF-Lausanne/18_May_2008 18]
[http://2008.igem.org/EPF-Lausanne/19_May_2008 19]	[http://2008.igem.org/EPF-Lausanne/20_May_2008 20]	[http://2008.igem.org/EPF-Lausanne/21_May_2008 21]	[http://2008.igem.org/EPF-Lausanne/22_May_2008 22]	[http://2008.igem.org/EPF-Lausanne/23_May_2008 23]	[http://2008.igem.org/EPF-Lausanne/24_May_2008 24]	[http://2008.igem.org/EPF-Lausanne/25_May_2008 25]
[http://2008.igem.org/EPF-Lausanne/26_May_2008 26]	[http://2008.igem.org/EPF-Lausanne/27_May_2008 27]	[http://2008.igem.org/EPF-Lausanne/28_May_2008 28]	[http://2008.igem.org/EPF-Lausanne/29_May_2008 29]	[http://2008.igem.org/EPF-Lausanne/30_May_2008 30]	[http://2008.igem.org/EPF-Lausanne/31_May_2008 31]

June
M	T	W	T	F	S	S
						[http://2008.igem.org/EPF-Lausanne/1_June_2008 1]
[http://2008.igem.org/EPF-Lausanne/2_June_2008 2]	[http://2008.igem.org/EPF-Lausanne/3_June_2008 3]	[http://2008.igem.org/EPF-Lausanne/4_June_2008 4]	[http://2008.igem.org/EPF-Lausanne/5_June_2008 5]	[http://2008.igem.org/EPF-Lausanne/6_June_2008 6]	[http://2008.igem.org/EPF-Lausanne/7_June_2008 7]	[http://2008.igem.org/EPF-Lausanne/8_June_2008 8]
[http://2008.igem.org/EPF-Lausanne/9_June_2008 9]	[http://2008.igem.org/EPF-Lausanne/10_June_2008 10]	[http://2008.igem.org/EPF-Lausanne/11_June_2008 11]	[http://2008.igem.org/EPF-Lausanne/12_June_2008 12]	[http://2008.igem.org/EPF-Lausanne/13_June_2008 13]	[http://2008.igem.org/EPF-Lausanne/14_June_2008 14]	[http://2008.igem.org/EPF-Lausanne/15_June_2008 15]
[http://2008.igem.org/EPF-Lausanne/16_June_2008 16]	[http://2008.igem.org/EPF-Lausanne/17_June_2008 17]	[http://2008.igem.org/EPF-Lausanne/18_June_2008 18]	[http://2008.igem.org/EPF-Lausanne/19_June_2008 19]	[http://2008.igem.org/EPF-Lausanne/20_June_2008 20]	[http://2008.igem.org/EPF-Lausanne/21_June_2008 21]	[http://2008.igem.org/EPF-Lausanne/22_June_2008 22]
[http://2008.igem.org/EPF-Lausanne/23_June_2008 23]	[http://2008.igem.org/EPF-Lausanne/24_June_2008 24]	[http://2008.igem.org/EPF-Lausanne/25_June_2008 25]	[http://2008.igem.org/EPF-Lausanne/26_June_2008 26]	[http://2008.igem.org/EPF-Lausanne/27_June_2008 27]	[http://2008.igem.org/EPF-Lausanne/28_June_2008 28]	[http://2008.igem.org/EPF-Lausanne/29_June_2008 29]
[http://2008.igem.org/EPF-Lausanne/30_June_2008 30]

July
M	T	W	T	F	S	S
	[http://2008.igem.org/EPF-Lausanne/1_July_2008 1]	[http://2008.igem.org/EPF-Lausanne/2_July_2008 2]	[http://2008.igem.org/EPF-Lausanne/3_July_2008 3]	[http://2008.igem.org/EPF-Lausanne/4_July_2008 4]	[http://2008.igem.org/EPF-Lausanne/5_July_2008 5]	[http://2008.igem.org/EPF-Lausanne/6_July_2008 6]
[http://2008.igem.org/EPF-Lausanne/7_July_2008 7]	[http://2008.igem.org/EPF-Lausanne/8_July_2008 8]	[http://2008.igem.org/EPF-Lausanne/9_July_2008 9]	[http://2008.igem.org/EPF-Lausanne/10_July_2008 10]	[http://2008.igem.org/EPF-Lausanne/11_July_2008 11]	[http://2008.igem.org/EPF-Lausanne/12_July_2008 12]	[http://2008.igem.org/EPF-Lausanne/13_July_2008 13]
[http://2008.igem.org/EPF-Lausanne/14_July_2008 14]	[http://2008.igem.org/EPF-Lausanne/15_July_2008 15]	[http://2008.igem.org/EPF-Lausanne/16_July_2008 16]	[http://2008.igem.org/EPF-Lausanne/17_July_2008 17]	[http://2008.igem.org/EPF-Lausanne/18_July_2008 18]	[http://2008.igem.org/EPF-Lausanne/19_July_2008 19]	[http://2008.igem.org/EPF-Lausanne/20_July_2008 20]
[http://2008.igem.org/EPF-Lausanne/21_July_2008 21]	[http://2008.igem.org/EPF-Lausanne/22_July_2008 22]	[http://2008.igem.org/EPF-Lausanne/23_July_2008 23]	[http://2008.igem.org/EPF-Lausanne/24_July_2008 24]	[http://2008.igem.org/EPF-Lausanne/25_July_2008 25]	[http://2008.igem.org/EPF-Lausanne/26_July_2008 26]	[http://2008.igem.org/EPF-Lausanne/27_July_2008 27]
[http://2008.igem.org/EPF-Lausanne/28_July_2008 28]	[http://2008.igem.org/EPF-Lausanne/29_July_2008 29]	[http://2008.igem.org/EPF-Lausanne/30_July_2008 30]	[http://2008.igem.org/EPF-Lausanne/31_July_2008 31]

August
M	T	W	T	F	S	S
				[http://2008.igem.org/EPF-Lausanne/1_August_2008 1]	[http://2008.igem.org/EPF-Lausanne/2_August_2008 2]	[http://2008.igem.org/EPF-Lausanne/3_August_2008 3]
[http://2008.igem.org/EPF-Lausanne/4_August_2008 4]	[http://2008.igem.org/EPF-Lausanne/5_August_2008 5]	[http://2008.igem.org/EPF-Lausanne/6_August_2008 6]	[http://2008.igem.org/EPF-Lausanne/7_August_2008 7]	[http://2008.igem.org/EPF-Lausanne/8_August_2008 8]	[http://2008.igem.org/EPF-Lausanne/9_August_2008 9]	[http://2008.igem.org/EPF-Lausanne/10_August_2008 10]
[http://2008.igem.org/EPF-Lausanne/11_August_2008 11]	[http://2008.igem.org/EPF-Lausanne/12_August_2008 12]	[http://2008.igem.org/EPF-Lausanne/13_August_2008 13]	[http://2008.igem.org/EPF-Lausanne/14_August_2008 14]	[http://2008.igem.org/EPF-Lausanne/15_August_2008 15]	[http://2008.igem.org/EPF-Lausanne/16_August_2008 16]	[http://2008.igem.org/EPF-Lausanne/17_August_2008 17]
[http://2008.igem.org/EPF-Lausanne/18_August_2008 18]	[http://2008.igem.org/EPF-Lausanne/19_August_2008 19]	[http://2008.igem.org/EPF-Lausanne/20_August_2008 20]	[http://2008.igem.org/EPF-Lausanne/21_August_2008 21]	[http://2008.igem.org/EPF-Lausanne/22_August_2008 22]	[http://2008.igem.org/EPF-Lausanne/23_August_2008 23]	[http://2008.igem.org/EPF-Lausanne/24_August_2008 24]
[http://2008.igem.org/EPF-Lausanne/25_August_2008 25]	[http://2008.igem.org/EPF-Lausanne/26_August_2008 26]	[http://2008.igem.org/EPF-Lausanne/27_August_2008 27]	[http://2008.igem.org/EPF-Lausanne/28_August_2008 28]	[http://2008.igem.org/EPF-Lausanne/29_August_2008 29]	[http://2008.igem.org/EPF-Lausanne/30_August_2008 30]	[http://2008.igem.org/EPF-Lausanne/31_August_2008 31]

September
M	T	W	T	F	S	S
[http://2008.igem.org/EPF-Lausanne/1_September_2008 1]	[http://2008.igem.org/EPF-Lausanne/2_September_2008 2]	[http://2008.igem.org/EPF-Lausanne/3_September_2008 3]	[http://2008.igem.org/EPF-Lausanne/4_September_2008 4]	[http://2008.igem.org/EPF-Lausanne/5_September_2008 5]	[http://2008.igem.org/EPF-Lausanne/6_September_2008 6]	[http://2008.igem.org/EPF-Lausanne/7_September_2008 7]
[http://2008.igem.org/EPF-Lausanne/8_September_2008 8]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/9_September_2008&action=edit 9]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/10_September_2008&action=edit 10]	[http://2008.igem.org/EPF-Lausanne/11_September_2008 11]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/12_September_2008&action=edit 12]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/13_September_2008&action=edit 13]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/14_September_2008&action=edit 14]
[http://2008.igem.org/EPF-Lausanne/15_September_2008 15]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/16_September_2008&action=edit 16]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/17_September_2008&action=edit 17]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/18_September_2008&action=edit 18]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/19_September_2008&action=edit 19]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/20_September_2008&action=edit 20]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/21_September_2008&action=edit 21]
[http://2008.igem.org/EPF-Lausanne/22_September_2008 22]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/23_September_2008&action=edit 23]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/24_September_2008&action=edit 24]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/25_September_2008&action=edit 25]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/26_September_2008&action=edit 26]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/27_September_2008&action=edit 27]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/28_September_2008&action=edit 28]
[http://2008.igem.org/EPF-Lausanne/29_September_2008 29]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/30_September_2008&action=edit 30]

October
M	T	W	T	F	S	S
		[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/1_October_2008&action=edit 1]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/2_October_2008&action=edit 2]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/3_October_2008&action=edit 3]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/4_October_2008&action=edit 4]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/5_October_2008&action=edit 5]
[http://2008.igem.org/EPF-Lausanne/6_October_2008 6]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/7_October_2008&action=edit 7]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/8_October_2008&action=edit 8]	[http://2008.igem.org/EPF-Lausanne/9_October_2008 9]	[http://2008.igem.org/EPF-Lausanne/10_October_2008 10]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/11_October_2008&action=edit 11]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/12_October_2008&action=edit 12]
[http://2008.igem.org/EPF-Lausanne/13_October_2008 13]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/14_October_2008&action=edit 14]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/15_October_2008&action=edit 15]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/16_October_2008&action=edit 16]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/17_October_2008&action=edit 17]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/18_October_2008&action=edit 18]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/19_October_2008&action=edit 19]
[http://2008.igem.org/EPF-Lausanne/20_October_2008 20]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/21_October_2008&action=edit 21]	[http://2008.igem.org/EPF-Lausanne/22_October_2008 22]	[http://2008.igem.org/EPF-Lausanne/23_October_2008 23]	[http://2008.igem.org/EPF-Lausanne/24_October_2008 24]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/25_October_2008&action=edit 25]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/26_October_2008&action=edit 26]
[http://2008.igem.org/EPF-Lausanne/27_October_2008 27]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/28_October_2008&action=edit 28]	[http://2008.igem.org/EPF-Lausanne/29_October_2008 29]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/30_October_2008&action=edit 30]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/31_October_2008&action=edit 31]

November
M	T	W	T	F	S	S
					[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/1_November_2008&action=edit 1]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/2_November_2008&action=edit 2]
[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/3_November_2008&action=edit 3]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/4_November_2008&action=edit 4]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/5_November_2008&action=edit 5]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/6_November_2008&action=edit 6]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/7_November_2008&action=edit 7]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/8_November_2008&action=edit 8]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/9_November_2008&action=edit 9]
[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/10_November_2008&action=edit 10]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/11_November_2008&action=edit 11]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/12_November_2008&action=edit 12]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/13_November_2008&action=edit 13]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/14_November_2008&action=edit 14]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/15_November_2008&action=edit 15]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/16_November_2008&action=edit 16]
[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/17_November_2008&action=edit 17]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/18_November_2008&action=edit 18]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/19_November_2008&action=edit 19]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/20_November_2008&action=edit 20]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/21_November_2008&action=edit 21]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/22_November_2008&action=edit 22]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/23_November_2008&action=edit 23]
[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/24_November_2008&action=edit 24]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/25_November_2008&action=edit 25]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/26_November_2008&action=edit 26]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/27_November_2008&action=edit 27]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/28_November_2008&action=edit 28]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/29_November_2008&action=edit 29]	[http://2008.igem.org/wiki/index.php?title=EPF-Lausanne/30_November_2008&action=edit 30]

Protocols

Reference : Most of these protocols come from http://openwetware.org

SOC medium : Super Original Catabolite Repressor

Bactotryptone 20g. Bacto-yeast extract 5g. NaCl 0.5g. 1M KCl 2.5ml ddH2O to 1000 ml Total Volume 1000ml Adjust pH to 7, with 10N NaOH. Autoclave to sterilize Add 20 ml of 1M glucose before use.

TE :

10xTE for 1 liter from stock solutions 10 ml 1M Tris-HCl pH 8.0 2 ml 0.5M EDTA pH 8.0 988 ml ddH2O

→ 10xTE is 10 mM Tris-HCl and 1 mM EDTA

   * For the Tris-HCl use Tris base and adjust to desired pH using HCl.

SOB : Super Original Broth

Used in growing bacteria for preparing chemically competent cells Ingredients

   * 0.5% (w/v) yeast extract
   * 2% (w/v) tryptone
   * 10 mM NaCl
   * 2.5 mM KCl
   * 20 mM MgSO4

Per liter:

   * 5 g yeast extract
   * 20 g tryptone
   * 0.584 g NaCl
   * 0.186 g KCl
   * 2.4 g MgSO4

!!! Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter.

CCMB80 buffer : For 1L

   * 10 mM KOAc pH 7.0 (10 ml of a 1M stock/L)
   * 80 mM CaCl2.2H2O (11.8 g/L)
   * 20 mM MnCl2.4H2O (4.0 g/L)
   * 10 mM MgCl2.6H2O (2.0 g/L)
   * 10% glycerol (100 ml/L)
   * adjust pH DOWN to 6.4 with 0.1N HCl if necessary
         o adjusting pH up will precipitate manganese dioxide from Mn containing solutions.
   * sterile filter and store at 4°C
   * slight dark precipitate appears not to affect its function

Antibiotics : For each antibiotic, solutions of 5 ml have been done and stored at -20°C (09.07.08)

Transformation

We used DH5-alpha cells from Bart's lab stock. We therefore followed the InVitrogen protocol.

Electrocompetent cells preparation From segall-lab protocol