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Team:University of Ottawa/11 July 2008
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Today in the Lab
Matt
- PTP2 Digestion
- Ran two digestion's of the PTP2 in parallel with one another: AatII/NcoI, EcoRV/NcoI. Neither Gel gave proper band sizes.
- PCR confirmation
- PCR confirmation of 97 plasmid showed up nicely on the gel, confirming that the integration was a success.
Chris
- Confirmation of AtCRE
- ran the 1% gel at 80 V for about forty minutes
- only primer bands showed after this time period
- it was suggested that phusion polymerase may not have been added before PCR, so 1 uL of phusion was added to the samples and phusion PCR was re-initiated
- following PCR, the results were run on a gel with the same results as above.
