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Team:University of Ottawa/30 July 2008
From 2008.igem.org
Today in the Lab
Tammy
| Sample | Concentration (ng/μL) | V (μL) | Total DNA (ng) |
| pSSA14 | 129 | 7 | 903 |
| OB | 170 | 6 | 1020 |
- Digestion Step 1 of pSSA14 with PstI
| Digestion Reaction components | Vol (μl) |
| H2O | 4 |
| Buffer 3 | 1.5 |
| BSA | 1.5 |
| PstI | 1 |
| DNA template | 7 |
| Total | 15 |
- Digestion Step 1 of OB with PstI
| Digestion Reaction components | Vol (μl) |
| H2O | 5 |
| Buffer 3 | 1.5 |
| BSA | 1.5 |
| PstI | 1 |
| DNA template | 6 |
| Total | 15 |
- Digestion Step 2 of pSSA14 with ClaI
| Digestion Reaction components | Vol (μl) |
| H2O | 9.5 |
| Buffer 4 | 3 |
| BSA | 1.5 |
| ClaI | 1 |
| DNA template | 15 |
| Total | 30 |
- Digestion Step 2 of OB with ClaI
| Digestion Reaction components | Vol (μl) |
| H2O | 9.5 |
| Buffer 4 | 3 |
| BSA | 1.5 |
| ClaI | 1 |
| DNA template | 15 |
| Total | 30 |
