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Team:UNIPV-Pavia/Notebook/Week1
From 2008.igem.org
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Notebook
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Week 1: 05/19/08 - May 05/23/08
05/19/08
Let’s start our IGEM 2008 experience!
At first, we broke the punch tool…:)
We used a scalpel to cut and resuspend the following 22 paper spots:
| BBa_I14032 | BBa_R0079 | BBa_R0062 | BBa_R0040 |
| BBa_R0082 | BBa_R0051 | BBa_J23100 | BBa_C0161 |
| BBa_C0062 | BBa_C0078 | BBa_C0179 | BBa_C0051 |
| BBa_C0012 | BBa_C0040 | BBa_I15010 | BBa_I15008 |
| BBa_I15009 | BBa_E0040 | BBa_E1010 | BBa_B0030 |
| BBa_B1006 | BBa_E0240 |
We used tweezers to put the cut paper into tubes containing 10 μl of warmed TE buffer.
We transformed 60 µl of TOP10 E. coli with 4 µl of DNA in TE for all 22 parts, plated transformed bacteria and incubated overnight at 37°C.
We used LB medium previously prepared, with the suitable antibiotic added.
