Wisconsin: Lignin Project/23 June 2008

Decided to use Herculase instead of TAQ in the PCR reactions (Herculase has proofreading ablitity)
Ran a new PCR reaction using the specific volumes designated by the Herculase designers.
No Bands formed.

Team Fungus:
Ran a touchdown PCR of Carbon and Nitrogen-limited cDNA using Herculase II polymerase
Ran PCR products through 0.9% agrose gel at 100V
Bands present for both at about 1.1kb