Wisconsin: Lignin Project/2 July 2008

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Team Sorbitol:

The pBAD30 was harvested using a Qiagen Miniprep kit and digested as described before.
We were able to extract enough plasmid DNA to perform a ligation.
We then set up a ligation with the following rations of vector to insert: 5:1, 3:1, 1:1, 1:3, 1:5, 1:0, 0:1
The mixture wasallowed to ligate at 4C overnight.

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