Wisconsin: Lignin Project/18 July 2008
From 2008.igem.org
Team Sorbitol
Froze down 3 400ul samples of RL257, MG1655, and JW3890 each containing pBAD30-srlD.
They were then stored at -80.
Primers were then designed and ordered to insert srlD into the pET28a vector which will provide a His tag that can be used to purify the enzyme.