Alberta NINT/31 July 2008
From 2008.igem.org
lab work (JD):
Counted colonies on both mine and Shannon's plates from yesterday's ligations -unfortunately equal numbers on negative plates and ligation plates so ligation did not work -my plate had 100s on the negative control so I assumed the vector was not cut properly Conclusion: Re-digest K102011 with more enzyme, for 2 hours and run very slowly on gel