Team:University of Ottawa/31 July 2008

From 2008.igem.org

Revision as of 14:51, 12 August 2008 by Djedrysiak (Talk | contribs)

Untitled Document

 

 

Contents

Today in the Lab

Chris

Microwave Digestion Experiment

  • Following protocol outlined online, tested a microwave protocol to hasten digestion period.
  • Digested DQ232597 with HindIII. Ran five samples: 5 seconds in micro, 10 seconds, 15 seconds, 10 seconds in and 2 minutes out (four times), water control, water control 10 seconds in microwave and 2 minutes out, 1 hr traditional digest.
  • Denatured HindIII at 80 C for 20 minutes, then ran products on a gel.
  • Results promising; however, more experimentation needed to confirm validity of this protocol

    • Matt

    Ligation

  • Another ligation of PTP2 insert with pSSA42 vector was performed with 3:1 ratio, 6:1 ratio, Vector with ligase, vector without ligase and H2O control.
  • 2 hr ligation was unsucessful, overnight ligation was usccessful for both 3:1 and 6:1.

    • Transformation

  • Transformed ligation product into competent cells - 3 different plates -3:1, 6:1 and control.

    • Dan

    Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/31_July_2008"