Team:Montreal/Notebook/August

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August 2008

August 12

  • Seeded the T9002 plasmid in BL21 A1s in the following way:

all tubes contain 3mL of medium

1. Control M9+Yeast +dextrose with Amp

2. T9002 colony 1 in LB +Amp

3. T9002 colony 2 in M9/Yeast + dextrose +Amp

4. T9002 colony 1 in M9/Yeast + dextrose +Amp

@ 9:15 PM

Colony 1 is in two tubes: M9 and LB. If the M9 is bad we'll know because it will have grown in LB. If it doesn't grow in either then something's up.