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Team:Montreal/Notebook/August
From 2008.igem.org
August 2008
August 12
- Seeded the T9002 plasmid in BL21 A1s in the following way:
all tubes contain 3mL of medium
1. Control M9+Yeast +dextrose with Amp
2. T9002 colony 1 in LB +Amp
3. T9002 colony 2 in M9/Yeast + dextrose +Amp
4. T9002 colony 1 in M9/Yeast + dextrose +Amp
@ 9:15 PM
Colony 1 is in two tubes: M9 and LB. If the M9 is bad we'll know because it will have grown in LB. If it doesn't grow in either then something's up.
August 13
- Diluted culture #2 of T9002 in M9/Yeast in three tubes of 11x, 22x and 44x.
