Team:Montreal/Notebook/August

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Contents

August 2008

August 1

  • Punched spot of J40001 into 15uL (should be 5uL) of TE buffer then performed following transformations:

1. 6uL of J40001 into TOP10 cells with amp

2. 2uL of pUC19 into TOP10 with amp (control)

3. 6uL of pGFP into MC4100 calls with amp

4. 6uL of J40001 into dH5alpha cells with amp

August 2

  • Removed plates with transformations from previous day from incubator. Growth on all, except very minimal on dH5alpha cells with J40001.


August 12

  • Prepared M9 Minimal media with 20mL of 20% w/v yeast broth, then autoclaved.
  • Seeded the T9002 plasmid in BL21 A1s in the following way:

all tubes contain 3mL of medium

1. Control M9+Yeast +dextrose with Amp

2. T9002 colony 1 in LB +Amp

3. T9002 colony 2 in M9/Yeast + dextrose +Amp

4. T9002 colony 1 in M9/Yeast + dextrose +Amp

@ 9:15 PM

Colony 1 is in two tubes: M9 and LB. If the M9 is bad we'll know because it will have grown in LB. If it doesn't grow in either then something's up.

August 13

  • Diluted culture #2 of T9002 in M9/Yeast in three tubes of 11x, 22x and 44x.
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