Alberta NINT/13 August 2008
From 2008.igem.org
lab work (JD):
Gel purification of TAIn_2, 3, 6, 7/X+H Collected Quantitative Data Performed Ligations of K102011+TAIn_2,3,6,7 Transformed XL1-B cells with ligations Plated on LB+kan35 Incubated at 37 overnight
lab work (SD):
Isolated DNA of TA2, TA3, TA6 and Ta7 from overnights using QIAquick spin miniprep standard protocol. Sequenced TA2, TA3, TA6 and TA7. Made overnights of the three colonies found on the pSB1A3 plate.