"
Team:Paris/Modeling/f5
From 2008.igem.org
and
so the expression
gives
and
and for calculated values of the TF,
and
| param | signification | unit | value |
| [expr(pFliA)] | expression rate of pFliA with RBS E0032 | nM.s-1 | see "findparam" need for 20 + 20 measures and 5x5 measures for the SUM? |
| γGFP | dilution-degradation rate of GFP(mut3b) | s-1 | ln(2)/3600 |
| [GFP] | GFP concentration at steady-state | nM | need for 20 + 20 measures and 5x5 measures for the SUM? |
| (fluorescence) | value of the observed fluorescence | au | need for 20 + 20 measures and 5x5 measures for the SUM? |
| conversion | conversion ration between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value |
| β5 | production rate of FlhDC-pFliA with RBS E0032 β5 | nM.s-1 | |
| (K3/{coefflhDC}n3) | activation constant of FlhDC-pFliA K3 | nMn3 | |
| n3 | complexation order of FliA-pFliA n3 | no dimension | |
| β13 | production rate of FliA-pFliA with RBS E0032 β13 | nM.s-1 | |
| (K9/{coeffliA}n9) | activation constant of OmpR-pFlhDC K9 | nMn9 | |
| n9 | complexation order of OmpR-pFlhDC n19 | no dimension |
Then, if we have time, we want to verify the expected relation
