Team:KULeuven/25 August 2008

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• The Team
  Research Labs and Groups Students Instructors Advisors Pictures
• The Project
  Summary Components

  Input Output Filter InverTimer Reset Cell Death Memory

  Safety End Evaluation Literature Brainstorm
• Ethics
  Introduction Three A's

  Defining the three A's Developments in modern physics Developments in biology

  Concerns & Issues

  Defining synthetic biology A framework Concerns Issues The need for answers

  Biological Robotics

  KULeuven iGEM 2008 project Biological robotics

  Afterword References
• Submitted Parts
  Sandbox
• Modeling
  Overview Kinetic Constants Components

  Output Filter InverTimer Reset Cell Death Memory

  Full Model Sensitivity Analysis Multi-cell Model Diffusion
• Data Analysis
  Overview New Parts

  GFP (LVA-tag) Other

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  Freezer Primers Ligation

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Lab Work

Wet Lab

• We did a PCR to test the transduction with donor and acceptor as control.
• Ligations were electroporated.
• The following parts were MiniPrepped: J23116+B0032, R0011+F1610, R0040+J23022, R0040+B0032, R0084+B0032. Then they were cut with EcoRI, SpeI and XbaI.
• The following ligations were set up: K145001+pSB1A2 and K145151+pSB1A2.

Dry Lab

Modeling

Further attempts at stochastic modeling of the filter using Matlabs SSA.

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