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User:University of Washington/26 August 2008
From 2008.igem.org
LuxR from AraC and TetR(Faifan)
ligation from gel extraction
-cultures of plasmid construct transformation ended up growing on Kan plates after incubating overnight at 36 degree Celsius
-selected 3 colonies from each plate(LuxR, GFP) and grew overnight to do sequencing
new ligation with CIP reaction
-miniprep I0462 x 3, ran gel and combined tubes
-restriction digest GFP and LuxR as in the table from yesterday(8/25)
-PCR purified digested promoter
-nanodropped the purified promoter: 261.9 ng/ul, 1.87(260/280), 2.04(260/230)
-did CIP reaction (Ingrid)
- mix 10X CIP Buffer + 0.5 units of CIP per ul/DNA + DNA(have final concentration of 0.5 ul/10 ul)
- incubated 37 degree Celsius 1 hour
- stored at -20
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