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Team:KULeuven/29 August 2008
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Lab Work
Wet Lab
- A liquid culture was made of the new parts and the ligations we did.
- We also did a colony PCR to test the ligations. They don't seem to be correct.
- Some more digests were made and purified out of gel.
- Ligations were set up.
Dry Lab
Had another good meeting today. Possibility was raised that adenosine methylation by the dam methylase is inhibiting our XbaI GATC restrictions. Checking it out. JM109 is probably dam+ so XbaI shouldn't cut, while for example JM110 and DM1 (Invitrogen) are dam-. If we want to use XbaI we'll need dam- cells.
Modeling
Nick continued his succesful work on multicellularity in MatLab. He even created a graph that looks like the blueprints of some modern building + created bacteria that could go back in time ;)
We were also able to show the relative effect of the filter by outputting some graphs in MatLab.
Wiki
All parameters on the wiki AND in the MatLab model have been checked and should be correct.
Remarks
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