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Project
Analysis
Construction
Characterization
Perspectives
← Yesterday
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October
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November
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Ligation L173 (
rbs-TetR
in
mRFP-LVA-tripart
-pSB1A3)
Name
Digestion number
Digested by
rbs-TetR
D112
EcoRI & SpeI (insert)
mRFP-LVA-tripart
D187
EcoRI & XbaI (vector)
Control
Sample
3:1
(insert:vector)
ratio
4:1
(insert:vector)
ratio
D112
(insert)
0 µL
1,4 µL
1,9 µL
D187
(vector)
2,5 µL
2,5 µL
2,5 µL
T4 DNA ligase 10X
buffer
2 µL
2 µL
2 µL
T4 DNA
ligase
1 µL
1 µL
1 µL
water
14,5 µL
13,1 µL
12,6 µL
"
