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User:University of Washington/5 September 2008
From 2008.igem.org
LuxR from AraC and TetR(Faifan)
- measured Absorbance of the overnight culture at 660 nm = 1.5
- diluted 60 ul culture into 5.40 ml Tsy+KAN. Absorbance at 660 nm = 0.035
- incubated 37 degree Celsius for 2.5 hrs, Absorbance at 600 nm = 0.7
- diluted 1 ml culture into 5 ml Tsy+Kan, OD@600nm = 0.165
- set up 96 well plate reader experiment. The total volume in each well was 200 ul.
| Arabinose(%), 10%Arabinose added (ul) | |||||
|---|---|---|---|---|---|
| 0, 0 | 0.05, 1 | 0.1, 2 | 0.50, 10 | 1.00, 20 |
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