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Transforming into Bacillus subtillis
From 2008.igem.org
Revision as of 10:22, 10 September 2008 by Riachalder (Talk | contribs)
- Add 10µl plasmid to 400µl cells.
- Mix by inverting the tube 5 times.
- Incubate at 37°C for 60 minutes whilst shaking.
- Centrifuge tube at 13,000g for 2 minutes to pellet the cells.
- Remove and discard 300µl supernatent. Re-centrifuge if pellet has already resuspended.
- Pipette remaining liquid and pellet up and down to resuspend.
- Plate onto agar.
