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Team:BCCS-Bristol/Calendar-Notebook/22 July 2008
From 2008.igem.org
Bead experiment 1
It was started using the beads, aspartate and bacteria in 0.3 % agar within the glass slide chambers. One bead was focused under the microscope; it didn't move after 30 minutes probably due to the observation that the bacteria were in the bottom layer and the beads on top of the motility medium in didn`t seem to get in contact. The chamber was left under the microscope overnight (results see 23 July 2008).
BioBrick Transformation
Carried on with biobrick transformation of the [http://partsregistry.org/Part:BBa_E0240 GFP generator]. The DNA was transformed into E. coli DH5α cells along with pUC19 as a control using chemical competent cells and heat shock.
Swimming agar assay
The chemotaxis experiment was continued, set up gradient over 8 hours of diffusion and innoculated with both MC1000 and MG1655 in agar in wells as before ( 21 July 2008).
