Frozen glycerol stock

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Revision as of 15:42, 16 September 2008 by Riachalder (Talk | contribs)
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  • Take 2mls of ON culture and pipette into a sterilised cryo tube.
  • Add 0.5ml of autoclaved 50% glycerol solution
  • Vortex briefly to mix
  • Store at -80˚C