Michał K.:

1. Restriction digestion of pTrc99A-AID with HindIII and NcoI (1x Tango buffer).

2. DNA electrophoresis and isolation of DNA encoding AID from proper band (600 bp).

3. Restriction digestion of pMPM-T5 with HindIII and NcoI (1x Tango buffer), use of alkaline phosphatase (CIAP).

4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band (4250 bp).

5. Overnight ligation of isolated DNA fragments.

</html>