Michał K.:

1. Gel electrophoresis (friday PCR's) and DNA isolation from proper bands.

2. PCR - translation fusion: AID + T7 RNA-polymerase

Primers:

AIDlNrH and T7pXbSal

Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion

Annealing temperature: 55 °C

Annealing time: 4 minutes

20 cycles

3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polymerase)