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From 2008.igem.org

At the steady-state, we have

and

so the expression

gives

and

and for calculated values of the TF,

and

param	signification	unit	value
[expr(pFliL)]	expression rate of pFliL with RBS E0032	nM.s-1	see "findparam" need for 20 + 20 measures and 5x5 measures for the SUM?
γGFP	dilution-degradation rate of GFP(mut3b)	min-1	0.0198
[GFP]	GFP concentration at steady-state	nM	need for 20 + 20 measures and 5x5 measures for the SUM?
(fluorescence)	value of the observed fluorescence	au	need for 20 + 20 measures and 5x5 measures for the SUM?
conversion	conversion ration between fluorescence and concentration	nM.au-1	(1/79.429)

param	signification corresponding parameters in the equations	unit	value
β53	production rate of FlhDC-pFliL with RBS E0032 β53	nM.min-1
(K47/{coefflhDC})	activation constant of FlhDC-pFliL K47	nM
n47	complexation order of FlhDC-pFliL n47	no dimension
β54	production rate of FliA-pFliL with RBS E0032 β54	nM.min-1
(K48/{coeffliA})	activation constant of FliA-pFliL K48	nM
n48	complexation order of FliA-pFliL n48	no dimension

Then, if we have time, we want to verify the expected relation

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