Michał K:
1. PCR - translation fusion: AID + T7 RNA-polymerase - optymalization (temperature gradient 60°C - 80°C).

Primers:

AIDlNrH and T7pXbSal

Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion

Annealing time: 4 minutes

35 cycles

2. Gel electrophoresis.
3. Optimalization of PCR - translation fusion: AID + T7 RNA-polymerase - MgCl2 cocentration and number of cycles.

Primers:

AIDlNrH and T7pXbSal

Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion

Annealing time: 4 minutes

4. Gel electrophoresis.