Michał K.

1. Isolation of hypothetical pMPM-T5+AID plasmids from cultures inoculated on previous day.

2. Restriction digestion of plasmids with HindIII and NcoI (1xTango buffer) - construct control.

3. Gel electrophoresis - choice of proper clones (all checked colonies).

Michał L., Ewa, Marcin.

Overnight inoculation and induction:

Top10 + 0,5 mM IPTG

pMPMAID + Glc + 0,01% L-Ara

pTRCAID + Glc + 0,5 mM IPTG