Team:Warsaw/Calendar-Main/9 July 2008

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Preparation of constructs with OmpA protein fusions
1. Colony PCR on colonies from plates with transformations pACYC177+OmpA_omega and pACYC177+OmpA_alpha Primers used: OmpaL_N and OmpaP_link
2. Confirmed transformant colonies inoculated to liquid LB with kanamycin


Preparation of construct pKS with A protein
1. Colony PCR on colonies from plates with transformations pKS-A
Primers used: AL+SacI and AP+NotI
2. Confirmed transformant colonies inoculated to liquid LB with ampicillin

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