Michał K.:

1. Isolation of hypothetical pMPM-T5 with transcription fusion.

2. Isolation of hypothetical pMPM-T5 with translation fusion.

3. Control digestion of pMPM-T5 with transcription fusion with EcoRI and XhoI (2x Tango buffer).

4. Control digestion of pMPM-T5 with translation fusion with BamHI (BamHI buffer).

5. Control digestion of pMPM-T5 with translation fusion with EcoRI and XbaI (2x Tango buffer).

6. Choice of proper clones and preparation for sequencing.

Here we found the clone with translational fusion on pMPM. These are different isolations in each lane. We have been working with the AT6 ever since.
Digestion was conducted with BamHI