Michał L., Ewa, Marcin

Plasmid isolation from night culture of our transformants.
Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.
Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and EcoRI.
Control digest of pMPM-T5 + T7 RNA-polymerase with EcoRI.
Control digest of pMPM-T5 + T7 RNA-polymerase with BamHI and XhoI.
DNA gel electrophoresis of digestion products.
Picking out proper colonies of transformants.